|
|
Handbook of Multilevel Analysis
Автор: H. Goldstein Жанр: Springer Издательство: Springer Год: 2008 Страниц: 508 Дата загрузки: 12 апреля 20122010-10-13
|
|
This book presents the state of the art in multilevel analysis, with an emphasis on more advanced topics. These topics are discussed conceptually, analyzed mathematically, and illustrated by empirical examples. Multilevel analysis is the statistical analysis of hierarchically and non-hierarchically nested data. The simplest example is clustered data, such as a sample of students clustered within schools. Multilevel data are especially prevalent in the social and behavioral sciences and in the biomedical sciences. The chapter authors are all leading experts in the field. Given the omnipresence of multilevel data in the social, behavioral, and biomedical sciences, this book is essential for empirical researchers in these fields.
|
| |
|
Core Concepts in Data Analysis: Summarization, Correlation and Visualization
Автор: Boris Mirkin Жанр: Springer Издательство: Springer Год: 2011 Страниц: 412 Дата загрузки: 15 сентября 20162012-12-20
|
|
This text is orientated at a Computer Science/Engineering student or professional, supervised or not, who would like to concentrate in depth on a few ideas and methods giving a holistic perspective on the area rather than to see the overwhelming variety of tools and methods developed so far, which is provided by other texts and references without much looking at interrelations between methods.
|
| |
|
Molecular Cytogenetics: Protocols and Applications
Автор: Fan Yao-Shan Жанр: Springer Издательство: Springer Год: 2003 Страниц: 424 Дата загрузки: 10 декабря 20092005-05-22
|
|
Yao-Shan Fan and a panel of senior scientists and pioneering researchers describe in step-by-step style the leading FISH techniques and those molecular technologies beyond FISH available for diagnostic services in genetics and oncology. The methods include labeling FISH probes for DNA and RNA targets, fluorescence genotyping, CGH microarray, spectral karyotyping/multicolor FISH, and primed in situ labeling. There are also techniques for multicolor fiber FISH, multi-telomere FISH, prenatal diagnosis using maternal blood, and preimplantation diagnosis. Oncological methods include simultaneous fluorescence immunophenotyping and FISH for leukemia and lymphoma, HER2 amplification in breast cancer, and CAC/PAC for cancer cytogenetics.
|
| |
|
RT-PCR Protocols
Автор: Connell Joe Жанр: Springer Издательство: Springer Год: 2002 Страниц: 380 Дата загрузки: 23 декабря 20092005-09-24
|
|
A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.
|
| |
|
Apoptosis: Techniques and Protocols
Автор: LeBlanc Andrea C. Жанр: Springer Издательство: Springer Год: 2002 Страниц: 272 Дата загрузки: 27 октрября 20092004-05-24
|
|
In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.
|
| |
|
Affinity Chromatography: Methods and Protocols
Автор: Zachariou Michael Жанр: Springer Издательство: Springer Год: 2007 Страниц: 360 Дата загрузки: 7 октрября 20102010-04-29
|
|
Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, this second edition volume aims to provide the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. It contains state-of-the-art, brand new protocols, and provides step-by-step laboratory instructions for readily reproducible results.
|
| |
|
Nano-Optics
Автор: Kawata Satoshi Жанр: Springer Издательство: Springer Год: 2002 Страниц: 336 Дата загрузки: 12 сентября 20092006-03-26
|
|
A presentation of the most advanced application of optical near-field microscopy to studies of fine metallic structures and related surface plasmons.
|
| |
|
Heavy Metal Stress in Plants: From Biomolecules to Ecosystems
Автор: Prasad M.N.V. Жанр: Springer Издательство: Springer Год: 2004 Страниц: 462 Дата загрузки: 22 марта 20092006-07-11
|
|
Whilst heavy metal phytotoxicity has been known for more than a century, it is astonishing that interest in the effects of heavy metals on organisms has only recently received added attention. Research in the past years, however, has confirmed the immense damage by metal pollution to plants, the soil and ultimately to humans. This completely updated and enlarged second edition gives a state-of-the art review on both field and laboratory work. It deals with the various functional and ecological aspects of heavy metal stress on plants and outlines the scope for future research and the possibilities for remediation.
|
| |
|
A Practical Approach to Microarray Data Analysis
Автор: Berrar Daniel P. Жанр: Springer Издательство: Springer Год: 2003 Страниц: 384 Дата загрузки: 28 июня 20092004-12-30
|
|
The book addresses the requirement of scientists and researchers to gain a basic understanding of microarray analysis methodologies and tools. It is intended for students, teachers, researchers, and research managers who want to understand the state of the art and of the presented methodologies and the areas in which gaps in our knowledge demand further research and development. The book is designed to be used by the practicing professional tasked with the design and analysis of microarray experiments or as a text for a senior undergraduate or graduate level course in analytical genetics, biology, bioinformatics, computational biology, statistics and data mining, or applied computer science.
|
| |
|
A Guide to Protein Isolation
Автор: Dennison C. Жанр: Springer Издательство: Springer Год: 2003 Страниц: 249 Дата загрузки: 16 марта 20092005-05-24
|
|
The logic of the overall approach to protein isolation is explained and the physical principles of each separation method are made clear by the use of simple models and analogies drawn from everyday experiences. The author's aim has been to deepen the readers' insight into protein isolation methods, so that they may tackle new problems and perhaps devise new approaches to old problems. Many of the methods described are drawn from the author's own research and are thus uniquely described here; examples are three-phase partitioning, non-linear electrophoresis and a simple approach to buffer making. In this 2nd edition, the treatment of the basic physical principles has been expanded and clarified, the importance of ionic strength in measuring enzyme pH optima is emphasised and a computer program for the calculation of buffers of defined ionic strength is provided. The section on three-phase partitioning has been expanded to include the latest research findings on the use of t-butanol to inhibit enzymes and minimise homogenisation artefacts, the treatment of HPLC has been expanded and the most common practical methods are explained in detail in a new chapter. Additional study questions are provided, as are the answers to all study questions.
|
| |
|
Single Nucleotide Polymorphisms: Methods and Protocols
Автор: Kwok Pui-Yan Жанр: Springer Издательство: Springer Год: 2003 Страниц: 288 Дата загрузки: 13 февраля 20092006-04-22
|
|
Pui-Yan Kwok, MD, PhD, has assembled a comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
|
| |
|
Oxidative Stress and Antioxidant Protocols
Автор: Armstrong Donald Жанр: Springer Издательство: Springer Год: 2002 Страниц: 336 Дата загрузки: 12 сентября 20092006-03-26
|
|
Donald Armstrong updates and expands his highly praised Free Radical and Antioxidant Protocols (Humana, 1998) with a collection of new and valuable methods for evaluating the pertubations in cell function resulting from increased oxidative stress. Presented in a user-friendly, step-by-step format, these readily reproducible techniques cover both free radical-derived and antioxidant biomarkers. The methodologies demonstrated include ELISA, HPLC, infrared spectroscopy, gas chromatography-mass spectroscopy, immunoblotting, electroelution fractionation, isoelectric focusing, voltametry, and electron paramagnetic resonance imaging. Special emphasis is given to the separation of complex mixtures of plant antioxidants, soft drug design to protect from toxic oxidative metabolites, in vitro oxidation conditions, and correcting for random measurement error to improve statistical interpretation. A soon-to-be-published companion volume, Oxidants and Antioxidants: Ultrastructural and Molecular Biology Protocols (0-89603-851-3), contains state-of-the-art molecular and ultrastructural methods that expand the total number of protocols to 109 assays.
|
| |
|
In Situ Detection of DNA Damage: Methods and Protocols
Автор: Didenko Vladimir V. Жанр: Springer Издательство: Springer Год: 2002 Страниц: 336 Дата загрузки: 12 сентября 20092006-03-26
|
|
In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
|
| |
|
Biostatistical Methods
Автор: Looney Stephen W. Жанр: Springer Издательство: Springer Год: 2002 Страниц: 224 Дата загрузки: 08 марта 20092003-06-28
|
|
Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in molecular biology, and demonstrate how these methods can be used in the development of new markers for exposure to a risk factor or for disease outcomes. Major areas of application include microarray analysis, proteomic studies, image quantitation, genetic susceptibility and association, evaluation of new biomarkers, and power analysis and sample size.
|
| |
|
Phosphoinositides in Subcellular Targeting and Enzyme Activation
Автор: Stenmark Harald Жанр: Springer Издательство: Springer Год: 2004 Страниц: 210 Дата загрузки: 19 марта 20092006-07-24
|
|
Cells of the immune system are activated by a variety of stimuli that are derived from other cells, ingested material or from invading microorganisms. This issue of CTMI focuses on the mechanisms of phosphoinositide-mediated protein recruitment to intracellular membranes.
|
| |
|
Physiology and Biochemistry of Metal Toxicity and Tolerance in Plants
Автор: Prasad M.N. Жанр: Springer Издательство: Springer Год: 2002 Страниц: 448 Дата загрузки: 16 мая 20092004-10-23
|
|
The aim of this book is to give an overview of the most important aspects of physiological and biochemical basis for metal toxicity and tolerance in plants. The book is expected to serve as a reference to university and college teachers, students of plant sciences, environmental biology, environmental biotechnology, agriculture, horticulture, forestry, plant molecular biology, and genetics.
|
| |
|
Terraforming
Автор: Beech Martin Жанр: Springer Издательство: Springer Год: 2009 Страниц: 304 Дата загрузки: 7 декабря 20112012-09-14
|
|
This book proposes a sound and realistic exploration on the topic of terraforming. Often used as the narrative premise in science fiction novels, terraforming is the process by which an uninhabitable planet might be converted into one capable of supporting life. This book presents what is physically possible today and hints what might conceivably be put into practice in the next several hundred years.The author works within the realms of current technology and known physics, although speculation on future advancements inevitably enters the discussion. Introductory chapters establish why terraforming will be of great benefit to human kind, and also put in place the basic physical arguments necessary to the terraforming process. The following parts look at various proposals that have been made for terraforming the planets Mars and Venus. The book concludes with a glimpse to the much deeper future when humankind will explore and colonize the outer solar system and possibly the newly discovered exoplanets.
|
| |
|
Financial Economics
Автор: Hens Thorsten Жанр: Springer Издательство: Springer Год: 2010 Страниц: 384 Дата загрузки: 15 декабря 20122012-05-24
|
|
Financial economics is a fascinating topic where ideas from economics, mathematics and, most recently, psychology are combined to understand financial markets. This book gives a concise introduction into this field and includes for the first time recent results from behavioral finance that help to understand many puzzles in traditional finance. The book is tailor made for master and PhD students and includes tests and exercises that enable the students to keep track of their progress. Parts of the book can also be used on a bachelor level. Researchers will find it particularly useful as a source for recent results in behavioral finance and decision theory.
|
| |
|
Conflict and Catastrophe Medicine
Автор: Buma Adriaan Hopperus Жанр: Springer Издательство: Springer Год: 2009 Страниц: 664 Дата загрузки: 03 августа 20112011-10-31
|
|
This 2nd updated edition is an entry-level text covering all aspects of deployment, not just medical or health-related, for use by health professionals in a resource-constrained environment brought about by local conflict or natural disasters. Since the 1st edition (2002), there have been seismic changes following the terrorist assault on the Twin Towers and the Pentagon. The Middle East, the Far East and parts of Africa are convulsed by conflict, verging on civil war. Colossal natural disasters have since occurred — Tsunamis (SE Asia), Earthquakes (Iran, Turkey, and Pakistan), a Hurricane (Gulf of Mexico).The working environment for the prospective humanitarian volunteer has changed forever. The book covers preparation, organisation, logistics, treatment of major trauma and medical emergencies, and the problems of delivering medicine in hostile/remote environments. It bridges the fields of medicine, nursing, international relations, history, politics and economics and also touches on nutrition, infection, trauma, psychiatry, and psychological medicine, and training.
|
| |
|
Data Correcting Algorithms in Combinatorial Optimization
Автор: Goldengorin Boris Жанр: Springer Издательство: Springer Год: 2012 Страниц: 126 Дата загрузки: 18 мая 20152015-04-19
|
|
Data Correcting Approaches in Combinatorial Optimization focuses on algorithmic applications of the well known polynomially solvable special cases of computationally intractable problems. The purpose of this text is to design practically efficient algorithms for solving wide classes of combinatorial optimization problems. Researches, students and engineers will benefit from new bounds and branching rules in development efficient branch-and-bound type computational algorithms. This book examines applications for solving the Traveling Salesman Problem and its variations, Maximum Weight Independent Set Problem, Different Classes of Allocation and Cluster Analysis as well as some classes of Scheduling Problems. Data Correcting Algorithms in Combinatorial Optimization introduces the data correcting approach to algorithms which provide an answer to the following questions: how to construct a bound to the original intractable problem and find which element of the corrected instance one should branch such that the total size of search tree will be minimized. The PC time needed for solving intractable problems will be adjusted with the requirements for solving real world problems.
|
| |
